After absorption, prulifloxacin is metabolised by esterases to ulifloxacin, which inhibits the formation of superhelix, cell division and reproduction by interfering the DNA toposomerase.

本品为前体,性代谢产物通过阻碍DNA拓扑构酶使细菌DNA无法形成超螺,细菌细胞无法分裂繁殖。