Methods By means of bicolour fluorescence and flow cytometry (FCM) analysis, the results were compared during different lay-out time, hemolysis time and washing time.

方法用双抗体双色免疫荧光标记，流式细胞仪分析，对标本放置不同时间、溶血后洗涤前后。

In vitro antigen presentation assays, in vivo administration of the bromodeoxyuridine coupled to flow cytometry assays assessed intra-islet T cell activation in the absence or presence of B cells.

离体实验测定抗原呈递活性；在体动物实验，用溴脱氧尿苷处理后以流式细胞术检测B细胞（存在或不存在时）对胰岛内T细胞活性影响。