Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.
方法 标本组织块用冷消化法
离出尿路上皮细胞，培养于无血清生长培养基。
Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.
方法 标本组织块用冷消化法离出尿路上皮细胞，培养于无血清生长培养基。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
方法取大鼠胚
离成纤维细胞,别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
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